DAY 1 - First Day We Met Room 148 Bioreactor

EPISODE 1- THE DAY WE MET EACH OTHER

Date:    4.10.2010 (Monday)

Time:    1P.M

Place:   Room 148 PPTI

This was the first that we started our laboratory experiment of IBG 302 (Bioreactor Operation). The planned for this day that to introduce us to the bioreactor scale of 2L and the brand is Infors HT. The ratio height to diameter of this bioreactor is approximately 2.5 : 1. It is very advisable that the ratio of height is at least twice bigger to the diameter of bioreactor.

Before Mr. Shaman started his presentation on the bioreactor, he had told us about fermentation that mostly we had learnt in class.

This is Mr. Shaman from INFORS HT company

The detail informations about that bioreactor have been explained more detail. As for the first day he had show us how to unpack the bioreactor start from its box. After that, he showed how to set up that bioreactor. Personally, at first we thought that its hard to set up the bioreactor but....

Bioreactor after take out from the box

Bioreactor before finish the set up

Now we would like to introduce the compartments of the bioreactor, that Mr. Shaman already told us, and also some pictures of them.

Mr. Shaman introduce us the compartments of the bioreactor. This is the vessel and its holder

The function of the vessel is where the fermentation take place,  and the headspace for the vessel to operate at optimum condition is usually 20-30% of total volume. The function of the holder is to hold the vessel. 

Sample Device

Support Frame

Harvest Line

The function of the harvest line is to harvest the desired product and also for the disposal purpose.

Rotameter

The rotameter is the means of manually controlling the amount of air entering the vessel and for controlling the gas flow rate.

Reagent Bottles Holder

The function of this holder is where the reagent bottles are usually placed during the fermentation.

Reagent Bottles

The reagent bottles that usually needed during the fermentation take place are, reagent bottle for base, antifoam and acid, but in this experiment we do not need acid because we dealing with microorganism, which is yeast (Saccharomyces Cerevisiae) which usually will produce acid trough out the process

pO2 Probe

The pO2 electrode should already be connected to the bioreactor base unit which has been powered for at least 2 hours to ensure the electrode is polarized.

Peristaltic Pumps

These pump heads are removable and autoclavable. Silicone tubing connects the inlet side of the pump head (left) to the reagent bottle and the outlet (right) to the 2-way inlet fitting on the vessel top plate. Pumps for acid and base are supplied as standard along and antifoam plus the feed or harvest are optional.

Operational Amplifiers

Operating Panel

Exit and Inlet Gas 

Air Compressor

The function is to provide air during fermentation process.

Gun Fire Pistol

Utility Connection

Securing Plate

Securing Lock

pH Probe

Vessel Fittings and Accessories

Each Minifors which is supplied as a complete system will have a number of fittings, consumable item and useful accessories included.

at the fittings should include

An exit gas cooler

A sample device and pipe

A 3-way inlet

An innoculation port

A pH electrode ( p02 electrode and antifoam are options)

Consumables should include

Pressure tubing for water and air connections at rear and exit gas cooler

Air inlet and outlet filters, single use, inlet side is marked

Filters for reagent bottles and sample device

Here are some pictures of the Vessel fittings and accessories ~

Vessel Fittigs

The view of Vessel Fittings,
as we can see the accessories 

EPISODE 1 PART 2

MEDIA PREPARATION FOR INNOCULATION OF SACCHAROMYCES CEREVISAE 

After Mr. Shaman gave us some explanations about the components of the bioreactor and their functions, we had move to second part of our lab which was the preparation of media for the innoculation of Saccharomyces Cerevisiae, which is the yeast that we will use in this experiment. The media that we used in this experiment was YEPG medium which contain yeast extract (1%), Peptone (2%) and glucose (2%). The total medium that needed for the innoculation was 200ml, so the weight for yeast extract, peptone and glucose needed were :

Yeast Extract (1%) = 2g

Peptone (2%)          = 4g

Glucose (2%)           = 4g

and after that we dissolved them with distilled water.

Peptone

Glucose

Yeast Extract

After the medium was done, the media was then autoclaved before the innoculation of the yeast step was taken. Then, our lab for the first day is done.....