The Graph OD reading at the abs 600nm versus time
This graph shows the curve of Saccharomyces Cerevisiae growth. Based on the graph , the lag phase starts from at 12.25 to 2.50 . But this phase does not last long because it is only for the adaptation of the cells towards the culture environment.At this time, the level of oxygen pressure is still high as well as temperature and pH.
After that , the growth increase from 2.5 to 6.5.The increment at the log phase (or also known as stationary phase) is due to cells’ growth rapidly and also the cells begin to uptake the glucose and minerals .The cell reproduction has two types .Firstly , sexual spore production and secondly , asexual budding .
After the log phase it supposes to be exponential phase . However , the growth goes down at 7.5 to 8.5 . However, there are two assumptions here:
1. It might because of the glucose level decreased too much during the log phase due to the yeast consumption.
2. Besides, this might also because of the error during the sample is taken where the sample taken might have mixed with the previous sample and as a result, the glucose concentration changed during sampling.
At the peak of the graph, the consumption of glucose is at maximum. Then the cells will stated to die off. When the concentration of glucose is going down therefore the growth of cells decreased too due to more of cells death .
1. It might because of the glucose level decreased too much during the log phase due to the yeast consumption.
2. Besides, this might also because of the error during the sample is taken where the sample taken might have mixed with the previous sample and as a result, the glucose concentration changed during sampling.
At the peak of the graph, the consumption of glucose is at maximum. Then the cells will stated to die off. When the concentration of glucose is going down therefore the growth of cells decreased too due to more of cells death .
After that, as more of the cells are death, the glucose level is increasing again and as the glucose increase, the cell’s growth is increasing too.
Then, after the amount of cells are growing at the maximum rate, the amount of glucose becoming limited at 12.5 am.Therefore, more cells tend to die and then, the toxin will start to produce from the cells. And this can cause the growth rate is going down abruptly.
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